Bee Team guards can be found mostly from the end of each Chapter (for a total of 20 per chapter) as well as when you change their State (for an additional 20 = 40), but you may also find a few out in the Overworld (adding the final 8 = 48). The first two Bee Team guards will come from completing the first two chapters, as that will be all you have access to to start before Trowzer's Paywall.